Recently, in the treatment of skin aging, a quite agenda creating, plant stem cell technology, is used. Undifferentiated plant cells obtained from plant tissue under laboratory conditions are called plant stem cells due to their totipotent properties. Plant stem cell extracts are produced from different plant species can be situated on the markets such as raw materials that has characteristic features such as anti-aging, anti-wrinkle, under-eye bruises reducer and anti-photo-aging.
Mucilages are herbal ingredients commonly used in cosmetics in lubricants, emulsifiers, gelling agents, granulating agents, lubricants, suspending agents, skin soothing agents and recently using as a binding reagent. Plant mucilages, are complex form of polysaccharides containing hydrocolloids and sugar-uronic acids. Mucilages have polysaccharides containing high amounts of hydroxyl groups that provide high water holding capacity. Due to these characteristic features, they have a potential to be used as a moisturizer for skin products. It's not possible to meet the market demands of the cosmetics industry just by obtaining mucilage with classic methods in terms of plant production processes and crop yield. At this point, the mucilage produced by biotechnological methods will be a new achievement. There are some studies showing that, in callus cultures, mucilage can be produced over 5 times more than produced in seeds (Gupta et al., 2015).
Quince (Cydonia oblonga) is a plant species containing mucilage at 25% of the dry weight of its seeds. In a study, mucilage derived from quince seed can increase the number of human dermal fibroblasts, thereby it has been suggested to be used for wound healing (Ghafouri et al., 2015). It has been demonstrated in several studies with many different biological activities that Quince (Cydonia oblonga) extract can be used as an antibacterial, antidiabetic, antihemolytic, antiallergic, antitumor,and kidney protecting reagent. It has also been reported, quince extract derived from different tissues contains quercetin, kaempferol, routine and caffeoylquinic acid derivatives and therefore have high antioxidant activity (Silva et al., 2004; Oliveira et al. 2007; Khoubnasabjaf and Jouyb 2011; Oliveira et al., 2012).
In the project, callus obtained from quince will be used to set up cell suspension culture.
Cell suspensions will be utilized for;
1. Increased amounts of mucilage by artificial miRNA application.
2. Enhanced amount of secondary metabolites especially polyphenols by elicitor treatment.
With the elicitor-induced plant stem cell technology method, plant extracts to be obtained from quince suspension culture will contain plant secondary metabolites in a high amount of antioxidants. At this stage, antiaging product will be obtained that has high antioxidant properties. Also, miRNA-induced plant stem cell technology will be used to obtain large amounts of mucilage. By combining different technologies, product with increased water holding capacity, high moisturizing and antioxidant activity will be obtained.
Various in vitro and in vivo tests will be performed for investigating the antioxidant, anti-aging activities, moisturizing effects and security of usage on the skin of quince stem cell extract and mucilage mixture. As the last step, safety and efficacy proven moisturizer, plant-derived high-value product with the antioxidant and anti-aging activity will be imparted to the dermocosmetic sector.